Biochemistry Laboratory: Modern Theory and Techniques pdf

罗德尼·弗雷德里克·博耶 (Hope College) 名誉教授、著名生物化学教育家、美国生物化学与分子生物学学会会员 (1983 年至今) 于 2 月 28 日去世，享年 79 岁，患有亨廷顿氏病。
博耶于 1942 年 8 月 25 日出生于内布拉斯加州的奥马哈。 他在爱荷华州的韦斯特玛学院获得化学和数学学士学位，然后前往科罗拉多州立大学获得博士学位。 1970 年专注于物理有机化学。 在密歇根大学医学院安娜堡分校，他作为美国国立卫生研究院的博士后研究员继续他的研究，师从生物化学家 Minor J. Coon，后者是细胞色素 P-450 领域的先驱，后来担任 ASBMB 主席。 Boyer 在 Grand Valley 州立大学的化学系工作了两年，然后于 1974 年加入密歇根州霍兰德的霍普学院化学和生物化学系。他在霍普学院待了 26 年，于 1985 年晋升为教授，并担任 系主任六年。 在 1991 年的休假期间，他作为美国癌症协会学者在位于博尔德的科罗拉多大学与诺贝尔奖获得者汤姆切赫一起工作。
Boyer 在博士后期间对铁化学产生了兴趣，并继续研究铁蛋白铁的氧化和还原对各种蛋白质和酶（如脱铁铁蛋白和超氧化物歧化酶）以及植物酚类物质的影响。 除了研究，他还编写了《现代实验生物化学》等本科生教材，并在 2000 年从希望学院退休后继续写作。他还担任 ASBMB 附属期刊《生物化学与分子生物学》的副主编 教育。

Biochemistry Laboratory: Modern Theory and Techniques pdf 罗德尼博耶

"Biochemistry Laboratory: Modern Theory and Techniques" is a comprehensive guidebook for students and researchers in biochemistry. Written by Rodney F. Boyer, a renowned biochemist, the book is a thorough introduction to modern techniques used in biochemical research.

The book covers essential topics in biochemistry such as protein purification, enzymology, chromatography, and electrophoresis, along with the latest developments in these fields. The content is presented in a clear and concise manner, making it accessible to beginners as well as seasoned researchers.

Boyer begins the book by providing a brief overview of the history of biochemistry, followed by chapters on laboratory safety and equipment. The subsequent chapters delve into the core techniques of biochemistry such as centrifugation, spectrophotometry, and electrophoresis. Each chapter includes step-by-step instructions for performing experiments, along with examples of data analysis and interpretation.

The book also covers advanced techniques such as mass spectrometry, X-ray crystallography, and fluorescence resonance energy transfer (FRET), which have revolutionized the field of biochemistry in recent years. The author emphasizes the importance of understanding the underlying principles of each technique, enabling researchers to design and optimize experiments to suit their specific needs.

Furthermore, the book includes a chapter on bioinformatics, which covers the use of computer tools and databases in biochemistry research. This section provides an excellent introduction to the field of computational biochemistry and the importance of data analysis and interpretation.

Overall, "Biochemistry Laboratory: Modern Theory and Techniques" is an excellent resource for students and researchers in biochemistry. The book provides a thorough understanding of essential techniques, including the latest developments in the field. The author's clear and concise writing style and step-by-step instructions make the book accessible to beginners, while the inclusion of advanced techniques ensures its relevance to seasoned researchers. The book's focus on understanding the underlying principles of each technique ensures that readers can design and optimize experiments to suit their specific needs.